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| ECZ5081 | Drug Analysis in Biological Samples | 2+0+0 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of PHARMACEUTICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Gökçe ÖZTÜRK | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to teach the fundamental principles, methods, and applications used in the qualitative and quantitative analysis of drugs in biological samples. The course aims to enable students to acquire the knowledge, skills, and interpretive competence required for the preparation, analysis, and evaluation of biological samples. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Explains the basic principles of drug analysis in biological samples and describes the collection, storage, and preparation of different types of biological specimens. | 1 - 4 - 5 | 1, | | LO - 2 : | Defines the spectroscopic, chromatographic, and electrochemical methods used in the qualitative and quantitative analysis of drugs in biological samples, and explains the fundamental principles of these techniques. | 1 - 4 - 5 | 1, | | LO - 3 : | Plans the pre-analytical procedures for biological samples, identifies and applies the appropriate extraction method. | 1 - 4 - 5 | 1, | | LO - 4 : | Assesses potential error sources, contamination risks, and matrix effects in biological matrices, and suggests corrective strategies to minimize analytical errors. | 1 - 4 - 5 | 1, | | LO - 5 : | Evaluates the obtained analytical data and assesses the reliability of the results. | 1 - 4 - 5 | 1, | | LO - 6 : | Interprets recent developments related to the determination of drugs in biological samples by applying modern drug analysis approaches. | 1 - 4 - 5 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| This course covers the fundamental principles, methods, and applications used in the qualitative and quantitative analysis of drugs in biological samples. It addresses the definition, collection, storage, preparation, and pre-analytical processing of biological specimens, as well as common problems, contamination, and sources of error encountered in biological matrices. Furthermore, modern approaches for the determination of drugs in biological samples, including spectroscopic, chromatographic, and electrochemical analytical techniques, are examined. The course also includes the evaluation of analytical results, validation procedures, and the fundamentals of data interpretation. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | The purpose and importance of drug analysis in biological samples | | | Week 2 | Types and characteristics of biological samples | | | Week 3 | Principles of sampling, storage, and transportation of biological samples | | | Week 4 | Sample preparation and pre-analytical procedures | | | Week 5 | Control of matrix effects and analytical interferences | | | Week 6 | Sources of contamination and methods for error prevention | | | Week 7 | Fundamental principles of liquid-liquid extraction and solid-phase extraction | | | Week 8 | Protein precipitation and advanced extraction techniques | | | Week 9 | Midterm exam | | | Week 10 | Fundamental principles of spectroscopic analysis methods and their application in biological samples | | | Week 11 | Fundamental principles of chromatographic analysis methods and their application in biological samples | | | Week 12 | Fundamental principles of electrochemical analysis methods and their application in biological samples | | | Week 13 | Validity of analytical methods, calibration, and measurement uncertainty concepts | | | Week 14 | Evaluation of analytical results and fundamentals of data interpretation | | | Week 15 | Modern drug analysis approaches and recent developments in biological sample analysis | | | Week 16 | End-of-term exam | | | |
| 1 | Nancy A. Brunzel. İdrar ve Vücut Sıvılarının Analiz Prensipleri. Palme Yayınevi. 9786052821640 | | | 2 | Recai İnam. Instrumental Analysis Laboratory Manual. Palme Yayınevi. 9786052823989 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 15.11.2025 | 1 | 50 | | End-of-term exam | 16 | 05.01.2026 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 4 | 14 | 56 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 102 |
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