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| ECZ2050 | Biochemistry - II Laboratory | 0+0+3 | ECTS:2 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Compulsory | | Department | DEPARTMENT of PHARMACEUTICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of laboratory per week | | Lecturer | Dr. Öğr. Üyesi Şeyda KANBOLAT | | Co-Lecturer | Assoc. Prof. Burak BARUT | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Within the scope of this course; It is aimed that students learn the basic techniques in the biochemistry laboratory. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Students learn the basic techniques in the biochemistry laboratory. | 1 - 5 | 1,4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Within the scope of this course; It is aimed that students learn the basic techniques in the biochemistry laboratory. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introducing the materials used in the biochemistry laboratory | | | Week 2 | Amino Acids and Protein | | | Week 3 | Determination of Protein Amount by Spectrophotometric Method | | | Week 4 | Investigation of the Kinetics of Urease Enzyme | | | Week 5 | Qualitative Carbohydrate Determination | | | Week 6 | Hydrolysis of Starch, Hydrolysis of Glycogen and Fasting Blood Sugar | | | Week 7 | Anaerobic Glycolysis | | | Week 8 | Detection of Protein in the Urine | | | Week 9 | Midterm | | | Week 10 | Lipids (Determination of Cholesterol) | | | Week 11 | Ketone Bodies (Tests for digestive disorders) | | | Week 12 | Liver (Tests on Bilirubin Metabolism) | | | Week 13 | Protein Purification Techniques | | | Week 14 | Protein Purification Techniques | | | Week 15 | DNA Purification Techniques | | | Week 16 | Final Exam | | | |
| 1 | Küçük, M. ve Güner, S. 2011; Temel biyokimya laboratuvar teknikleri ve uygulamaları. Derya Kitabevi.
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| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 17.04.2025 | | 50 | | End-of-term exam | 16 | 11.06.2025 | | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Laboratuar çalışması | 3 | 14 | 42 | | Arasınav için hazırlık | 1 | 5 | 5 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 1 | 5 | 5 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 54 |
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