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| BIO 542 | Practical Appl.Res.Tech. in Biochemistry - II | 0+4+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL BIOCHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Lab work , Practical | | Contact Hours | 14 weeks - 4 hours of practicals per week | | Lecturer | Prof. Dr. Ahmet ALVER | | Co-Lecturer | Assoc. Prof. Dr. Ahmet ALVER | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The course aims to provide the students with a general knowledge on the theory of techniques used in biochemistry. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | know biochemical methods | 1,2,3,4 | 1,2,4 | | PO - 2 : | learn biochemical laboratory methods in human health | 1,2,3,4 | 1,2,4 | | PO - 3 : | apply biochemical methods | 1,2,3,4 | 1,2,4 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| ChromatographyElectrophoresis |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Presentation of Chromatographic systems in our laboratory | | | Week 2 | Choice of a colon | | | Week 3 | Establishing of retention time by HPLC | | | Week 4 | HPLC Application I | | | Week 5 | HPLC Application II | | | Week 6 | HPLC Application III | | | Week 7 | HPLC Application IV | | | Week 8 | Mid-term exam | | | Week 9 | Presentation of electrophoresis systems | | | Week 10 | SDS PAGE electrophoresis | | | Week 11 | Protein electrophoresis | | | Week 12 | Lipoprotein electrophoresis | | | Week 13 | Immunoelectrophoresis | | | Week 14 | Blotting | | | Week 16 | End-of-term exam | | | |
| 1 | Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California | | | 2 | Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois | | | 3 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | |
| 1 | Pingoud,A.,Urbanke,C,Hoggett,J.Jeltsch,A.2002; Biochemical Methods, Wiley-VCH,Weinheim | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 20 | | Laboratory exam | 12 | | 4 | 30 | | End-of-term exam | 17 | | 3 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 5 | 14 | 70 | | Arasınav için hazırlık | 2 | 14 | 28 | | Arasınav | 2 | 1 | 2 | | Klinik Uygulama | 4 | 5 | 20 | | Dönem sonu sınavı için hazırlık | 3 | 14 | 42 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 221 |
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