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| HIS5130 | Cytochemical and histochemical tecniques | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of HISTOLOGY and EMBRYOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | - | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | make the students learn Cytochemical and histochemical tecniques |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Be able to explain Lipid histochemistry and How to show lipids
| 1,2,4 | 1,4 | | PO - 2 : | Be able to tell silvering method and Kromaffin reaction | 1,2,4 | 1,4 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Lipid histochemistry and diagnosis
How to show lipids
oil-soluble dyes
Sudan Black method for lipids
Oil red-O
Ozmium tetraoksit
Kalsiyum için Alizarin red S
silvering method for Gold
Grimelius silver method for Arjirofil cells (especially pancreatic A cells)
Silver method for decreasing cell Arjirofil
Kromaffin reaction
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Lipid histochemistry and diagnosis | | | Week 2 | How to show lipids | | | Week 3 | oil-soluble dyes
| | | Week 4 | Sudan Black method for lipids | | | Week 5 | Oil red-O
| | | Week 6 | Ozmium tetraoksit
| | | Week 7 | Alizarin red S for calcium | | | Week 8 | Mid-term exam | | | Week 9 | silvering method for Gold | | | Week 10 | Grimelius silver method for Arjirofil cells (especially pancreatic A cells)
| | | Week 11 | Decreasing Silver method for Arjirofil cell | | | Week 12 | Laboratory Exam | | | Week 13 | Kromaffin reaction | | | Week 14 | Immunocytochemistry related electron microscopy | | | Week 16 | End-of-term exam | | | |
| 1 | Bancroft JD, Cook HC, Stirling, RW, Turner DR. Manual of Histological Techniques and Their Diagnostic Application, Churchill Livingstone, Edinburgh, 1994. | | | |
| 1 | Ozban N., Özmutlu Ö. Mikropreparasyon Yöntemleri. İ.Ü. Fen Fakültesi Basımevi, İstanbul, 1991. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Laboratory exam | 12 | | 1 | 20 | | End-of-term exam | 16 | | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 13 | 26 | | Laboratuar çalışması | 2 | 13 | 26 | | Arasınav için hazırlık | 1 | 13 | 13 | | Arasınav | 1 | 1 | 1 | | Ödev | 4 | 12 | 48 | | Kısa sınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 16 | 32 | | Total work load | | | 147 |
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