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| EBK5201 | Research Methods in Biochemistry | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of BIOCHEMISTRY (PHARMACY) | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Burak BARUT | | Co-Lecturer | Prof. Rezzan Aliyazıcıoğlu
Prof. Oktay Yıldız
Assoc. Prof. Arzu Özel
Assoc. Prof. Sermet Yıldırmış | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To teach the basic methods used to make research in biochemistry |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | recognizes and uses materials and reagents used in biochemistry | 2,3,7 | 1,3 | | PO - 2 : | recognizes and uses pH meters | 2,3,7 | 1,3 | | PO - 3 : | rives information about centrifugation and use it | 2,3,7 | 1,3 | | PO - 4 : | recognizes spectrophotometer and can measure | 2,3,7 | 1,3 | | PO - 5 : | give information about electrophoresis
| 2,3,7 | | | PO - 6 : | use chromatographic methods | 2,3,7 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Glass and plastic materials used in the laboratory, to obtain pure water, the purity of reagent chemicals, analytical techniques and general concepts, potentiometric techniques, pH meters, centrifugation techniques, Spectrophotometry, Electrophoresis, Nephelometry, Chromatography and Separation mechanisms, biochemical systems commonly used chromatography techniques and uses of these techniques
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction of glass and plastic materials used in the laboratory
| | | Week 2 | Information about pure water, and production methods | | | Week 3 | The purity of reagent chemicals and standards | | | Week 4 | Potentiometric methods | | | Week 5 | Theory and types of centrifugation | | | Week 6 | Spectrophotometric methods | | | Week 7 | Protein electrophoresis | | | Week 8 | Midterm | | | Week 9 | DNA electrophoresis | | | Week 10 | Western blotting | | | Week 11 | Types of chromatographic methods
| | | Week 12 | Column chromatography | | | Week 13 | Ion exchange chromatography | | | Week 14 | PCR | | | Week 15 | CRISPR | | | Week 16 | Final Exam
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| 1 | CariA. Burtis, Edward R. Ashwood, Tietz Texbookof Clinical Chemistry,WB Saunders company A Division of Harcourt and Company London,1999 | | | |
| 1 | Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois | | | 2 | Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California | | | 3 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 10/11/2021 | 1 | 50 | | End-of-term exam | 16 | 16/01/2022 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 14 | 3 | 42 | | Sınıf dışı çalışma | 10 | 8 | 80 | | Laboratuar çalışması | 4 | 2 | 8 | | Arasınav için hazırlık | 4 | 4 | 16 | | Arasınav | 3 | 1 | 3 | | Ödev | 5 | 4 | 20 | | Dönem sonu sınavı için hazırlık | 3 | 6 | 18 | | Total work load | | | 187 |
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