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| EBK5202 | Research methods in biochemistry-II | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of BIOCHEMISTRY (PHARMACY) | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Group study, Lab work | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Rezzan Aliyazıcıoğlu, Sermet Yıldırmış | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To introduce advanced methods used in biochemistry |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | use HPLC | 1,2,8,11 | 1,3 | | PO - 2 : | use the methods of electrophoresis | 1,2,4,9,10,11 | 1,3 | | PO - 3 : | use immunoassays | 1,2,10,11 | 1,3 | | PO - 4 : | use PCR | 1,2,8,10,11 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| High-pressure liquid chromatography (HPLC), gas chromatography, immunoassay methods, merits, Enzimimmunoassay, Radioimmunoassay, the general concepts of electrophoresis and separation mechanisms, types of electrophoresis, PCR, Western blotting, and biochemical systems uses of these techniques |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Electrophoresis methods | | | Week 2 | Types and use of electrophoresis | | | Week 3 | chromatographic methods and column chromatography | | | Week 4 | Ion Exchange Chromatography | | | Week 5 | affinity Chromatography | | | Week 6 | Adsorption Chromatography | | | Week 7 | High pressure liquid chromatography | | | Week 8 | Mid-term exam | | | Week 9 | High pressure liquid chromatography | | | Week 10 | gas Chromatography | | | Week 11 | gas Chromatography | | | Week 12 | immunoassays | | | Week 13 | immunoassays | | | Week 14 | PCR reactions | | | Week 15 | PCR reactions | | | Week 16 | Final exam | | | |
| 1 | ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects- D. M. Kemeny (Editor), S. J. Challacombe (Editor) | | | 2 | Practical High-Performance Liquid Chromatography, 4th Edition-Veronika R. Meyer GC/MS: A Practical User's Guide, Marvin C. McMaster, Christopher Master | | | |
| 1 | Tietz Textbook of Clinical Chemistry, Carl A. Burtis, Edward R. Ashwood.WB Saunders Company, 3rd Edition, 1999 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 12/04/2014 | 2 | 50 | | End-of-term exam | 16 | 06/06/2014 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 14 | 3 | 42 | | Arasınav için hazırlık | 2 | 2 | 4 | | Arasınav | 1 | 2 | 2 | | Uygulama | 5 | 3 | 15 | | Dönem sonu sınavı için hazırlık | 5 | 2 | 10 | | Dönem sonu sınavı | 1 | 2 | 2 | | Total work load | | | 75 |
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